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Title: Run silent, run deep
Type of Resource: Vídeo
Objective: To show the yolk sac of a killifish embryo that contains a population of deep cells which migrate through the subepithelial space
Abstract: Presents the yolk sac of a killifish embryo contains a population of deep cells which migrate through the subepithelial space, and this sequence follows one such cell crawling within the normal matrices and cell substrates of an intact embryo. At the two-cell stage, this Fundulus heteroclitus embryo was injected with a GFP-actin plasmid. Expression is mosaic, and the non-expressing cells of the embryo act as a backdrop for the dramatic migrations of these wandering cells. If you watch carefully, you can see a few cells expressing very low amounts of fluorescent actin, giving context to the fact that this deep cell is not migrating on glass. This sequence begins as a deep cell extends a large protrusion out of the plane of focus, and the cell body can be seen to pour into the leading edge. This cell migrates with a structured lamellipodium, which shows retrograde waves of actin as the cell progresses. A thin cortical band of labeled actin can be seen around the entire periphery. The leading edge cytoplasm is clearly distinct from the rest of the cell material
Observation: Duration: 26 s. Contributors: Pat Wadsworth (University of Massachusetts at Amherst, Amherst, MA). Work conducted by RF at Mount Holyoke College, South Hadley, M. A. To visualize this source its necessary the instalation of Quick time. Available at: <http://www.apple.com/pt/quicktime/>
Curriculum Component: Educação Superior::Ciências Biológicas::Morfologia
Theme: Educação Superior::Ciências Biológicas::Morfologia::Citologia e Biologia Celular
Author: Fink, Rachel
Language: Not Applicable (NA)
Country: United States (us)
Publisher: The American Society for Cell Biology, ASBC Image & Video Library
Web Address: http://cellimages.ascb.org/cdm4/item_viewer.php?CISOROOT=/p4041coll12&CISOPTR=235&CISOBOX=1&REC=3
Date: 2007-10
Rightsholder: The American Society for Cell Biology. ASBC Image & Video Library
License: You may use the Images only for research and educational purposes in an educational institution. Images may be stored on a network server, hard drive or removable media, but only while this Agreement remains in effect. All of the Images you use must be accompanied by a notice stating, "© [Date and Author's or Owner's Name]. All rights reserved. Reprinted under license from The American Society for Cell Biology." The notice shall appear with the Images or on a credits page. "Educational purposes" means (i) non-commercial instruction or curriculum based teaching by educators to students at nonprofit educational institutions, (ii) planned non-commercial study, investigation or research directed toward making a contribution to a field of knowledge, or (iii) presentation of research findings at non-commercial peer conferences, workshops or seminars, but in any event there must be express written permission from ASCB before an Image can be published. "Educational institutions" include K-12 schools, colleges and universities. Libraries, museums, hospitals, research institutes and other nonprofit organizations are considered educational institutions when they engage in nonprofit instructional, research or scholarly activities for educational purposes
Submitter: Universidade de Brasília (UnB)
URI: http://objetoseducacionais2.mec.gov.br/handle/mec/23065
This Educational Object appears in the following Types of resources: Educação Superior: Ciências Biológicas: Morfologia: Vídeos